Ultrastructural study of the effects of cyclosporine in the brainstem of Wistar rats submitted to the ethidium bromide demyelinating model

Arquivos de Neuro-Psiquiatria

Eduardo Fernandes Bondan²  Maria Anete Lallo²  Dominguita Lühers Graça¹ 

[1] ,Universidade PaulistaSão Paulo SP ,Brazil

关键词: central nervous system;    myelin sheath;    oligodendroglia;    Schwann cells;    cyclosporine;    ethidium bromide;    sistema nervoso central;    bainha de mielina;    células de Schwann;    oligodendróglia;    ciclosporina;    brometo de etídio;   

DOI  :  10.1590/S0004-282X2008000300019

来源: SciELO

【 摘 要 】

The ethidium bromide-demyelinating model (EB) was used to study remyelination in the brainstem under the use of cyclosporine (CsA). Wistar rats were submitted to intracisternal injection of 0.1% EB or 0.9% saline solution, and others were taken as histologic controls (group I). Within those injected with EB, some have not received immunosuppressive treatment (II); some were treated by intraperitonial route with CsA (III.E - 10 mg/kg/day). Rats from group III.C were injected with saline solution and treated with CsA. The animals were perfused from 15 to 31 days post-injection collecting brainstem sections for light and transmission electron microscopy studies. After EB injection it was noted the presence of macrophages and non-degraded myelin debris, demyelinated axons, oligodendrocyte or Schwann cell remyelinated axons, groups of infiltrating pial cells, hypertrophic astrocytes and few lymphocytes. Tissue repair of EB-induced lesions in group III.E was similar to that of group II, but with the presence of a higher density of oligodendrocytes near remyelinating areas.

【 授权许可】

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