Cytological features of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation

Arquivos Brasileiros de Oftalmologia

Jeison De Nadai Barros²  Myrna Serapião Dos Santos²  Telma Regina Maria Pereira Barreiro²  Rubens Belfort Jr²  José Álvaro Pereira Gomes¹ 

[1] ,Universidade Federal de São Paulo Department of Ophthalmology São Paulo SP ,Brazil

关键词: Stem cells;    Cytological techniques;    Limbus corneae;    Goblet cells;    Epithelial cells;    Living donors;    Transplantation;    autologous;    Células-tronco;    Técnicas citológicas;    Limbo da córnea;    Células caliciformes;    Células epiteliais;    Doadores vivos;    Transplante autólogo;   

DOI  :  10.1590/S0004-27492011000400003

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate by impression cytology (IC) the corneal surface of live limbal tissue donor eyes for autograft or allograft limbal stem cell transplantation (LSCT). METHODS: Twenty limbal donors were enrolled (17 for autograft LSCT and 3 for allograft). Impression cytology was performed before transplantation of superior and inferior limbal grafts and after the third postoperative month. RESULTS: Impression cytology analysis showed sheets of corneal epithelial cells and goblet cell absence beyond the edge of the keratectomy sites in all patients, suggesting that conjunctival invasion towards the center did not occur in any eye. Partial conjunctivalization within 2 to 3 clock hours, confirmed by the presence of goblet cells, was limited to the keratectomy site in 10% of the cases. CONCLUSION: A clear central corneal surface was demonstrated in all eyes following surgery leading to the conclusion that limbal donation was a safe procedure in this group of patients. A small percentage of eyes can have donor sites re-epithelized with conjunctival cells at the periphery of the cornea.

【 授权许可】

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