期刊论文详细信息
Arquivos Brasileiros de Oftalmologia
Cytokeratin expression in corneal dystrophies
Anamaria Baptista Coutinho2  Denise De Freitas2  João Pessoa De Souza Filho2  Zélia Maria S. Corrêa1  Alexandre Nakao Odashiro2  Miguel N. Burnier Jr1 
[1] ,São Paulo Federal UniversitySão Paulo SP ,Brazil
关键词: Corneal dystrophies;    hereditary;    Keratins;    Immunohistochemistry;    Cornea;    Distrofias hereditárias da córnea;    Queratinas;    Imuno-histoquímica;    Córnea;   
DOI  :  10.1590/S0004-27492011000200010
来源: SciELO
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【 摘 要 】

PURPOSE: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. METHODS: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on pa- raffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). RESULTS: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. CONCLUSION: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.

【 授权许可】

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