Cytokeratin expression in corneal dystrophies

Arquivos Brasileiros de Oftalmologia

Anamaria Baptista Coutinho²  Denise De Freitas²  João Pessoa De Souza Filho²  Zélia Maria S. Corrêa¹  Alexandre Nakao Odashiro²  Miguel N. Burnier Jr¹ 

[1] ,São Paulo Federal UniversitySão Paulo SP ,Brazil

关键词: Corneal dystrophies;    hereditary;    Keratins;    Immunohistochemistry;    Cornea;    Distrofias hereditárias da córnea;    Queratinas;    Imuno-histoquímica;    Córnea;   

DOI  :  10.1590/S0004-27492011000200010

来源: SciELO

【 摘 要 】

PURPOSE: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. METHODS: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on pa- raffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). RESULTS: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. CONCLUSION: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.

【 授权许可】

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