A rapid polymerase chain reaction protocol to detect adenovirus in eye swabs

Arquivos Brasileiros de Oftalmologia

Rodrigo Melo Mendes²  Maurício Lacerda Nogueira²  João Trindade Marques²  Maria Valéria Corrêa Pereira¹  Maria Amélia De Souza Machado²  Anderson Saporetti Cunha¹  Fernando Cansado Trindade¹  Erna Geessien Kroon² 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de Microbiologia

关键词: Polymerase chain reaction;    Adenoviruses;    human;    Keratoconjunctivitis;    Adenovirus infections;    human;    Eye infections;    viral;    Herpesviridae infections;    Reação em cadeia da polimerase;    Adenovirus humano;    Ceratoconjuntivite;    Infecções humanas por adenovirus;    Infecções oculares virais;    Infecções por herpesviridae;   

DOI  :  10.1590/S0004-27492004000300010

来源: SciELO

【 摘 要 】

PURPOSE: Viruses of the Adenoviridae family are associated with many clinical syndromes, possessing 50 serotypes. These agents and viruses of the Herpesviridae family are the two major agents responsible for viral conjunctivitis, and a rapid diagnosis is important due to the epidemic character of adenoviral infections. METHODS: We developed a PCR without DNA extraction for adenovirus using primers that amplify a 300 bp fragment of the hexon capsid protein gene from many serotypes. RESULTS: Swab samples from cornea of seven patients with keratoconjunctivitis were analyzed, and one of them was PCR positive for adenovirus. The sequence of this fragment shows a 100% homology with the sequence of adenovirus type 8. CONCLUSION: Sequencing of 300 bp from the hexon gene allows to identify almost all Ad serotypes, including all serotypes related to epidemic keratoconjunctivitis (8,19,37) and almost all serotypes involved with Ad-associated conjunctivitis.

【 授权许可】

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