Effects of FGF-2 and EGF removal on the differentiationof mouse neural precursor cells

Anais da Academia Brasileira de Ciências

Telma T. Schwindt²  Fabiana L. Motta²  Barnabé Gabriela F.²  Massant Cristina G.²  Alessander O. Guimarães¹  Maria Elisa Calcagnotto²  João B. Pesquero¹  Luiz E. Mello² 

[1] ,Universidade Federal de São Paulo Departamento de Fisiologia São Paulo SP ,Brasil

关键词: neural precursor cells;    neurosphere;    fibroblast growth factor 2;    epidermal growth factor;    differentiation;    células precursoras neurais;    neuroesfera;    fator de crescimento de fibroblasto 2;    fator de crescimento epidérmico;    diferenciação;   

DOI  :  10.1590/S0001-37652009000300009

来源: SciELO

【 摘 要 】

Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in suspension showed different patterns of neuronal localization (core versus shell) for both EGF and FGF-2 withdrawal and control groups. Taken together, these results show that EGF and FGF-2 removal play an important role in NPC differentiation and may contribute to a better understanding of mechanisms of NPC differentiation. Our findings suggest that depriving NPC of growth factors prior to grafting might enhance their chance to effectively integrate into the host.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License