(The) roles of anoctamin chloride channels regulating embryonic neurogenesis

anoctamin;CaCC;embryonic neurogenesis;neurosphere;610

융합과학기술대학원 분자의학 및 바이오제약학과 ;

University:서울대학교 대학원

关键词: anoctamin;    CaCC;    embryonic neurogenesis;    neurosphere;    610;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/133321/1/000000004087.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

The embryonic neurogenesis which new neurons are generatedin the embryonic brainis critical for the development of the central nervous system. Abnormal neurogenesis often results in some fatal defects in the brain development as well as psychological disorders.As neurons become mature,the decrease ofintracellular chloride ionconcentrationin the embryonic neurons determinestransmembrane potential of neuronal membrane, which regulates actions of GABAergic transmission in the early brain development. Although the concentration of intracellular chloride ion is actively determinedin the embryonic neurodevelopmentbychloride transporters, NKCC1 and KCC2,it remains clearly unknown about how Cl-permeable chloride channels are involved in the embryonic CNS development. The anoctamin family (ANOs) is endogenous calcium activated chloride channels with various physiological functions. With the fact that Ca2+ signals, widely known as a second messenger,generated duringthe embryonic neurogenesismediate calcium activated chloride channels, we therefore investigated whether anoctamins are required for the embryonic neurogenesis. Using real time quantitative-PCR technique, we determined the level of mRNAs of various anoctamin channels as embryonic neural ii stem/progenitor cells increase the size of neurosphere.As a result, we found that mRNA levels of ANO3, ANO6, ANO8, and ANO10 were increased as the size of neurospheres enlarged. Especially, ANO8 was extensively increased by about 1000-fold. In addition, we identified ANO8 expression pattern in the developing brain. Moreover, we performed the knock down assay of ANO8for proliferation assay and migration assay. Then, we determined that ANO8 had influence on cellular migration, not on proliferation.Taken together, these results suggest that ANO3, ANO6, ANO8, and ANO10 play important roles in embryonic neural stem/progenitorcells.In particular, ANO8 have been strongly connectedto embryonic neurogenesis. This study provides insights into molecular mechanisms underlying chloride channels are involved in the embryonic neurodevelopment.