In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms

Brazilian Journal of Oral Sciences

Bruna Paloma De Oliveira¹  Carla Cabral Dos Santos Accioly Lins¹  Fátima Alves Diniz¹  Liliane Lima Melo¹  Célia Maria Machado Barbosa De Castro¹ 

关键词: endodontics;    Enterococcus faecalis;    methylene blue;    microbiology;    photodynamic therapy;   

DOI  :  10.1590/1677-3225v13n1a11

来源: SciELO

【 摘 要 】

AIM: To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT). METHODS: The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (10 8 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane's comparisons. RESULTS: The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001). CONCLUSIONS: PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.

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