Inhibition of osteoblast activity by zoledronic acid

Jornal Brasileiro de Patologia e Medicina Laboratorial

Fernanda Gonçalves Basso²  Ana Paula Silveira Turrioni¹  Josimeri Hebling¹  Carlos Alberto De Souza Costa¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Odontologia de Piracicaba

关键词: osteonecrosis;    repair;    mineralization;    osteoblasts;    osteonecrose;    reparo;    mineralização;    osteoblastos;   

DOI  :  10.1590/S1676-24442013000500011

来源: SciELO

【 摘 要 】

INTRODUCTION: Patients treated with nitrogen-containing bisphosphonates, such as zoledronic acid (ZA), have frequently shown oral bone exposure areas, termed osteonecrosis. In addition, these patients may also present low repair and regeneration potential, mainly after tooth extractions. These side-effects caused by bisphosphonates may be due to their inhibitory effects on oral mucosa and local bone cells. OBJECTIVE: To evaluate the effects of ZA on the mineralization capacity of cultured osteoblasts. MATERIALS AND METHODS: Human immortalized osteoblasts (SaOs-2) were grown in plain culture medium (Dulbecco's Modified Eagle Medium [DMEM] + 10% fetal bovine serum [FBS]) in wells of 24-well plates. After 48-hour incubation, the plain DMEM was replaced by a solution with ZA at 5 µM which was maintained in contact with cells for seven, 14 or 21 days. After these periods, cells were evaluated regarding alkaline phosphatase (ALP) activity and mineral nodule formation (alizarin red). Data were statistically analyzed by Mann-Whitney test, at 5% of significance level. RESULTS: ZA caused significant reduction on ALP activity and mineral nodules formation by cultured osteoblasts in all evaluated periods (p < 0.05). CONCLUSION: These data indicate that ZA causes inhibition on the osteogenic phenotype of cultured human osteoblasts, which, in turn, may reduce bone repair in patients subjected to ZA therapy.

【 授权许可】

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