STUDIES REGARDING THE IDENTIFICATION OF SOME HISTO-MORPHOLOGICAL PARAMETERS THAT COULD BE USED AS MARKERS FOR BONE MINERALIZATION APPRECISAL IN PIGS – SCIENTIFIC SUPPORT IN OSTEOPOROSIS STUDY

Scientific Papers Animal Science and Biotechnologies

GABI DUMITRESCU¹  D. DRINCEANU¹  LAVINIA ŞTEF² 

[1] Faculty of Animal Sciences and Biotechnologies, Timisoara;Faculty of Animal Sciences and Biotechnologies, Timisoarar;

关键词: osteoporosis;    mineralization;    osteoblasts;    osteoclasts;    osteoid;    histomorphometry;   

DOI  :  

来源: DOAJ

【 摘 要 】

The modern approach of the post-menopause osteoporosis etiology relies on the unbalance between the processes of bone resorption and formation, caused by the lack of estrogen hormones which determine different combined forms of increasing/decreasing the osseous cell activity, osteoblasts and osteoclasts by type. With regards to this, some histomorphometric analyses are carried out in order to determine the mineralized bone and osteoid relative quantity, and also the activity of cells having a formation function (osteoblasts) or a resorption of the osseous tissue (osteoclasts). American Society for Bone and Mineral Research recommend the evaluation of the following histomorphometric parameters: the volume of the osseous trabeculae (BV/TV, % - the percentage of the osseous tissue in a certain volume. Schematically, it represents the report full/empty within the bone); osteoid area (OS/BS, % - the percentage of the surface of the bone formed on a certain bone area); osteoblast area (Ob. S/BS, % - the percentage of the spongy bone trabecular and span areas with morphologically-active osteoblasts); osteoid volume (OV/BV, % - the osteoid percentage within a certain bon volume); osteoid width (O. Th, m, - the average width of the osteoid tape, which represents the support for calcification); osteoclast area (Oc. S/BS, % - the percentage of the trabecular areas with resorption gaps occupied by one or many osteoclasts, considering the fact that calcium deficiency causes the increase of osteoclast number and stimulates their activity); mineralization rate through apposition (MAR, m/day), calculated in divisions per average distance between two fluorescent tagging (it results from calceine accumulation at the mineralization front level). This double tagging has been accomplished through calceine i.m. injection at intervals of 6 days, 48 hours before sample taking. The researches presented in this paper work belong to the subcontract CEEX no. 110-2, partner no. 2, within the contract CEEX 110 entitled "Experimental model for the study upon the bioavailability of some nutritional factors (Ca, B, phytosterols) and their influence upon bone mineralization in pigs, a scientific support in the study upon osteoporosis".

【 授权许可】

Unknown