Immunomodulatory effects of oral antidiabetic drugs in lymphocyte cultures from patients with type 2 diabetes

Jornal Brasileiro de Patologia e Medicina Laboratorial

Karina Faccio Mello²  Adroaldo Lunardelli²  Márcio Vinícius Fagundes Donadio²  Eduardo Caberlon²  Carmen Silvana Araújo De Oliveira²  Carolina Maria Alves Bastos²  Melissa Guerra Simões Pires²  Fernanda Bordignon Nunes²  Jarbas Rodrigues De Oliveira¹ 

[1] ,Pontifícia Universidade Católica do Rio Grande do Sul

关键词: Diabetes mellitus;    Chlorpropamide;    Metformin;    Immunomodulation;    Diabetes mellitus;    Clorpropamida;    Metformina;    Imunomodulação;   

DOI  :  10.1590/S1676-24442011000100006

来源: SciELO

【 摘 要 】

INTRODUCTION AND OBJECTIVE: It has been suggested that type 2 diabetes is an inflammatory response manifestation. The main drugs used to treat type 2 diabetes are sulphonylureas and biguanides. The aim of this study was to demonstrate the modulatory effects of oral hypoglycemic drugs (chlorpropamide and metformin) on lymphocyte proliferation in vitro and ex vivo. METHODS: Peripheral blood mononuclear cells were isolated from human blood by gradient centrifugation. T-lymphocytes were stimulated by phytohemagglutinin (PHA) and oral hypoglycemic drugs. RESULTS: In both in vitro and ex vivo experiments, there was a reduction in cell proliferation after treatment with oral hypoglycemic drugs. When both drugs were used in combination, a high level of cytotoxicity was observed, which made analysis of immunomodulatory effects unfeasible. DISCUSSION AND CONCLUSION: We demonstrated that diabetes itself may reduce cell proliferation significantly when stimulated by PHA, which may indicate that diabetic patients have difficulties in promoting an efficient inflammatory response. Moreover, the use of oral hypoglycemic drugs may aggravate this situation.

【 授权许可】

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