Jornal Brasileiro de Patologia e Medicina Laboratorial | |
Immunomodulatory effects of oral antidiabetic drugs in lymphocyte cultures from patients with type 2 diabetes | |
Karina Faccio Mello2  Adroaldo Lunardelli2  Márcio Vinícius Fagundes Donadio2  Eduardo Caberlon2  Carmen Silvana Araújo De Oliveira2  Carolina Maria Alves Bastos2  Melissa Guerra Simões Pires2  Fernanda Bordignon Nunes2  Jarbas Rodrigues De Oliveira1  | |
[1] ,Pontifícia Universidade Católica do Rio Grande do Sul | |
关键词: Diabetes mellitus; Chlorpropamide; Metformin; Immunomodulation; Diabetes mellitus; Clorpropamida; Metformina; Imunomodulação; | |
DOI : 10.1590/S1676-24442011000100006 | |
来源: SciELO | |
【 摘 要 】
INTRODUCTION AND OBJECTIVE: It has been suggested that type 2 diabetes is an inflammatory response manifestation. The main drugs used to treat type 2 diabetes are sulphonylureas and biguanides. The aim of this study was to demonstrate the modulatory effects of oral hypoglycemic drugs (chlorpropamide and metformin) on lymphocyte proliferation in vitro and ex vivo. METHODS: Peripheral blood mononuclear cells were isolated from human blood by gradient centrifugation. T-lymphocytes were stimulated by phytohemagglutinin (PHA) and oral hypoglycemic drugs. RESULTS: In both in vitro and ex vivo experiments, there was a reduction in cell proliferation after treatment with oral hypoglycemic drugs. When both drugs were used in combination, a high level of cytotoxicity was observed, which made analysis of immunomodulatory effects unfeasible. DISCUSSION AND CONCLUSION: We demonstrated that diabetes itself may reduce cell proliferation significantly when stimulated by PHA, which may indicate that diabetic patients have difficulties in promoting an efficient inflammatory response. Moreover, the use of oral hypoglycemic drugs may aggravate this situation.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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