Determination of HPV DNA viral load by hybrid capture assay and its association with cytological findings

Jornal Brasileiro de Patologia e Medicina Laboratorial

Inês Aparecida Tozetti²  Ilzia Doraci Lins Scapulatempo²  José Eduardo Levi¹  Antonio Walter Ferreira¹ 

[1] ,Universidade Federal de Mato Grosso do Sul Departamento de Patologia

关键词: Human papillomavirus;    Viral load;    Cytology;    Papilomavírus humano;    Carga viral;    Citologia;   

DOI  :  10.1590/S1676-24442006000600008

来源: SciELO

【 摘 要 】

OBJECTIVE: To compare the relation between HPV viral load by hybrid capture II test (HCII) and cytological findings. METHODS: Three hundred sixty-two reagent samples to HPV DNA by HCII had their viral loads classified in four categories and correlated to cytological results. RESULTS: Twenty-two samples (6.1%) were reagent only to low-risk oncogenic types (group A) and 340 (93.9%) were reagent to high-risk oncogenic types (group B). The correlation between viral load for the reagent samples to group A and cytological results showed low-grade squamous intraepithelial lesion (LSIL) predominance (50%). Most of this group samples had viral load between 1 to <10RLU/PCA. Of the patients that were reagent to group B 52.1% had LSIL cytology and 38.2% were negative to intraepithelial lesion and malignancy (NILM) cytology. The patients with LSIL had viral load well distributed with a slight predominance of 100 to < 1,000RLU/PCB category. The samples had viral load between 1 to <10RLU/PCB showed NILM cytology predominance (48.1%). High-grade squamous intraepithelial lesions (3.4%) were present on the samples with viral load between 100 to <1,000RLU/PCB (p = 0.023). There was a correlation between the median for group B viral load and LSIL/HSIL results. CONCLUSIONS: The quantification of viral load, mainly of high-risk HPV types, may be a useful tool for dealing with patients who have suspicious lesions.

【 授权许可】

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