Growth of Paecilomyces variotii in B0 (diesel), B100 (biodiesel) and B7 (blend), degradation and molecular detection

Brazilian Journal of Biology

J Gassen¹  Fm Bento¹  Apg Frazzon¹  Mf Ferrão¹  Iv Marroni¹  Ab Simonetti¹ 

关键词: biodiesel;    Paecilomyces variotii;    deteriogenic;    biodegradation;    infrared spectroscopy;    PCR;    biodiesel;    Paecilomyces variotii;    deteriogênico;    biodegradação;    espectroscopia de infravermelho;    PCR;   

DOI  :  10.1590/1519-6984.15613

来源: SciELO

【 摘 要 】

AbstractThe introduction of biodiesel to diesel may allow the fuel to be more susceptible to microorganism growth, especially during incorrect storage. To evaluate the effect of adding biodiesel in pure diesel on the growth of Paecilomyces variotii, microcosms containing pure diesel (B0), blend diesel/biodiesel (B7) and pure biodiesel (B100) were used. In microcosm with minimal mineral medium and B0, B7 or B100, after 60 days, the biomass (dry weight) formed at interface oil-water in B7 and B100 was significantly higher when compared to that of B0. Infrared analysis showed reduction of the carbonile fraction in B7 and B100 suggesting formation of intermediate compounds in B7. To monitor possible contamination of fuel storage tank by P. variotii samples were collected and analysed by specific-PCR assay for detection of P. variotii spores in the aqueous phase. This method was able to detect a minimum of 103 spores ml–1, corresponding to 0.0144 ng µl–1 of DNA. Specificity was tested against Aspergillus fumigatus and Pseudallescheria boydii.

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