Anatomy and lignification status of the Lophanthera lactescens Ducke plant grown in vitro

Brazilian Journal of Biology

D. Amaral²  Hs. Abreu²  Hrp. Lima¹  Kv. Gevú¹  Tf. Elias²  J. Tenório² 

[1] ,Universidade Federal Rural do Rio de Janeiro Instituto de Florestas Departamento de Produtos FlorestaisSeropédica RJ ,Brazil

关键词: lignification;    fluorescence;    infrared microspectroscopy;    lignificação;    fluorescência;    microscopia infravermelho;   

DOI  :  10.1590/S1519-69842012000200019

来源: SciELO

【 摘 要 】

This aim of this study was to evaluate the lignification in young stems of the Lophanthera lactescens Ducke plant grown in vitro L. lactescens (Malpighiaceae), a species endemic in the Brazilian Amazon that possesses both medicinal properties and could be used in the forest product industry. Plants grown in vitro condition in MS medium were analyzed using Infrared (IR) microspectroscopy in a diffuse reflectance mode, fluorescence microscopy. Moreover, histochemical tests such as the Wiesner and Maüle test were run to monitor the process of lignification in cell walls. The spectra of IR microscopy recorded using cross section tissue are representative of guaiacyl/syringyl lignin, based on the 1336 and 1246 cm-1 signal. Individuals presenting stem sprains, resulting from the marked development, produced gelatinous fibers with a clear cellulose layer. Initially, fluorescence microscopy demonstrated lignin deposition in the cell corner region having progressive deposition in the secondary wall of tracheary elements of the stem.

【 授权许可】

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