Effect of histological processing and methacrylate sectioning on the area of gill tissue in teleost

Brazilian Journal of Biology

Al. Cruz²  Mn. Fernandes²  Sf. Perry¹ 

[1] ,Universidade Federal de São Carlos Departamento de Ciências Fisiológicas São Carlos SP ,Brazil

关键词: gills;    morphometry;    methacrylate;    shrinkage;    armored catfish;    brânquias;    morfometria;    metacrilato;    redução de área branquial;    cascudo;   

DOI  :  10.1590/S1519-69842009000200022

来源: SciELO

【 摘 要 】

Deformation of biological tissues may occur during histological processing and results in loss of accuracy when quantitative information about cells, tissues and organs is necessary. In this study, the gill tissue from armored catfish (Pterygoplichthys anisitsi) was quantified in each step of processing using the stereological principles. During processing for glycol methacrylate embedding, gill tissue from shrinks significantly but regains its original dimensions after sectioning.

【 授权许可】

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