Bacterial Growth Kinetics under a Novel Flexible Methacrylate Dressing Serving as a Drug Delivery Vehicle for Antiseptics

International Journal of Molecular Sciences

Christina Forstner¹  Johannes Leitgeb²  Rupert Schuster²  Verena Dosch⁴  Axel Kramer⁶  Keith F. Cutting⁵  David J. Leaper³ 

[1] Department of Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria; E-Mail:;Department for Trauma Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria; E-Mails:;Faculty of Medical Sciences, Newcastle University, NE1 7RU Newcastle upon Tyne, UK; E-Mail:;Department for Hospital Hygiene, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria; E-Mail:;Faculty of Society and Health, Buckinghamshire New University, WD3 5TG Uxbridge, UK; E-Mail:;Institute for Hygiene and Environmental Medicine, University Medicine Greifswald, Walther-Rathenau Strasse 49a, 17489 Greifswald, Germany; E-Mail:

关键词: wound dressing;    biofilm;    drug delivery;    antiseptic;    polyhexanide;    povidone-iodine;    octenidine-dihydrochloride;    methacrylate;    Altrazeal;    Prontosan C;    Octenisept;    Braunol;   

DOI  :  10.3390/ijms140510582

来源: mdpi

【 摘 要 】

A flexible methacrylate powder dressing (Altrazeal^®) transforms into a wound contour conforming matrix once in contact with wound exudate. We hypothesised that it may also serve as a drug delivery vehicle for antiseptics. The antimicrobial efficacy and influence on bacterial growth kinetics in combination with three antiseptics was investigated in an in vitro porcine wound model. Standardized in vitro wounds were contaminated with Staphylococcus aureus (MRSA; ATCC 33591) and divided into six groups: no dressing (negative control), methacrylate dressing alone, and combinations with application of 0.02% Polyhexamethylene Biguanide (PHMB), 0.4% PHMB, 0.1% PHMB + 0.1% betaine, 7.7 mg/mL Povidone-iodine (PVP-iodine), and 0.1% Octenidine-dihydrochloride (OCT) + 2% phenoxyethanol. Bacterial load per gram tissue was measured over five days. The highest reduction was observed with PVP-iodine at 24 h to log₁₀ 1.43 cfu/g, followed by OCT at 48 h to log₁₀ 2.41 cfu/g. Whilst 0.02% PHMB resulted in a stable bacterial load over 120 h to log₁₀ 4.00 cfu/g over 120 h, 0.1% PHMB + 0.1% betaine inhibited growth during the first 48 h, with slightly increasing bacterial numbers up to log₁₀ 5.38 cfu/g at 120 h. These results indicate that this flexible methacrylate dressing can be loaded with various antiseptics serving as drug delivery system. Depending on the selected combination, an individually shaped and controlled antibacterial effect may be achieved using the same type of wound dressing.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland