Genetic analysis of Melipona quadrifasciata Lep. (Hymenoptera: Apidae, Meliponinae) with RAPD markers

Brazilian Journal of Biology

A. M. Waldschmidt²  P. Marco-junior¹  E. G. Barros¹  L. A. O. Campos¹ 

[1] ,Universidade Estadual do Sudoeste da Bahia Departamento de Ciências Biológicas Jequié BA ,Brazil

关键词: Hymenoptera;    stingless bees;    Melipona quadrifasciata;    RAPD;    genetic distance;    Hymenoptera;    abelhas sem ferrão;    Melipona quadrifasciata;    RAPD;    distância genética;   

DOI  :  10.1590/S1519-69842002000500022

来源: SciELO

【 摘 要 】

Melipona quadrifasciata ("mandaçaia") can be subdivided into two subspecies: M. q. anthidioides and M. q. quadrifasciata. In the present study we used RAPD markers to estimate intercolonial genetic variation among 69 colonies of Melipona quadrifasciata. Ten workers per colony were analyzed. The intercolony genetic distances based on RAPD markers ranged from 29.5% (colonies collected in the State of São Paulo vs colonies from the State of Minas Gerais) to 34.2% (São Paulo vs Santa Catarina). These results indicate a high genetic similarity among the colonies analyzed.According to the genetic distances two different groups could be distinguished. The first containing the samples from Santa Catarina region and the second, samples from Paraná, São Paulo, Minas Gerais, and Espírito Santo. Based on the molecular analysis, bees belonging to the different subspecies M. q. quadrifasciata (from Santa Catarina) and M. q. anthidioides (from the other regions) were distinguished.

【 授权许可】

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