Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns

Genetics and Molecular Biology

Rogério O. Souza²  Geraldo Moretto¹  Maria C. Arias¹  Marco A. Del Lama² 

[1] ,Universidade Federal de São Carlos Departamento de Genética e Evolução São Carlos SP ,Brazil

关键词: subspecies differentiation;    Melipona quadrifasciata;    cytochrome b;    mtDNA;    PCR-RFLP patterns;   

DOI  :  10.1590/S1415-47572008000300009

来源: SciELO

【 摘 要 】

Melipona quadrifasciata quadrifasciata and M. quadrifasciata anthidioides are subspecies of M. quadrifasciata, a stingless bee species common in coastal Brazil. These subspecies are discriminated by the yellow stripe pattern of the abdominal tergites. We found Vsp I restriction patterns in the cytochrome b region closely associated to each subspecies in 155 M. quadrifasciata colonies of different geographical origin. This mitochondrial DNA molecular marker facilitates diagnosis of M. quadrifasciata subspecies matrilines and can be used to establish their natural distribution and identify hybrid colonies.

【 授权许可】

CC BY   
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