Binding effect of proline-rich-proteins (PRPs) on in vitro antimicrobial activity of the flavonoids

Brazilian Journal of Microbiology

Jawaad Ahmed Ansari¹  Shahina Naz¹  Omer Mukhtar Tarar¹  Rahmanullah Siddiqi¹  Muhammad Samee Haider¹  Khalid Jamil¹ 

关键词: proline rich proteins;    flavonoids;    catechin;    myrecetin;    kaempferol;    pelargonidin;    cyanidin;    gelatin;    casein;    binding constants;   

DOI  :  10.1590/S1517-838246120130280

来源: SciELO

【 摘 要 】

The interaction of the cyanidin, pelargonidin, catechin, myrecetin and kaempferol with casein and gelatin, as proline rich proteins (PRPs), was studied. The binding constants calculated for both flavonoid-casein and flavonoid-gelatin were fairly large (105–107 M−1) indicating strong interaction. Due to higher proline content in gelatin, the binding constants of flavonoid-gelatin (2.5 × 105–6.2 × 107M−1) were found to be higher than flavonoid-casein (1.2 × 105–5.0 × 107 M−1). All the flavonoids showed significant antibacterial activity against the tested strains. Significant loss in activity was observed due to the complexation with PRPs confirming that binding effectively reduced the concentration of the free flavonoids to be available for antibacterial activity. The decline in activity was corresponding to the values of the binding constants. Though the activities of free catechin and myrecetin were higher compared to pelargonidin, cyanidin and kaempferol yet the decline in activity of catechin and myrecetin due to complexation with casein and gelatin was more pronounced.

【 授权许可】

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