Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain

Brazilian Journal of Microbiology

Alessandra Smaniotto²  Aline Skovronski²  Elisandra Rigo²  Siu Mui Tsai¹  Ademir Durrer¹  Lillian Liva Foltran¹  Natália Paroul²  Marco Di Luccio¹  J. Vladimir Oliveira¹  Débora De Oliveira¹  Helen Treichel¹ 

[1] ,Universidade Regional Integrada do Alto Uruguai e das Missões Departamento de Engenharia de Alimentos Erechim RS ,Brazil

关键词: Sporidiobolus pararoseus;    'synthetic lipase';    submerged fermentation;    enzyme characterization;   

DOI  :  10.1590/S1517-83822014000100043

来源: SciELO

【 摘 要 】

Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP' enzymes presented high activities after exposure to pH 6.5 and 40 ºC. On the other hand, the 'IC' and 'IP' extracts kept their activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary studies of application of the crude lipase extract in the enzymatic production of geranyl propionate using geraniol and propionic acid as substrates in solvent-free system led to a reaction conversion of 42 ± 1.5%.

【 授权许可】

CC BY-NC   
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