'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation

Brazilian Journal of Microbiology

Alessandra Smaniotto¹  Aline Skovronski¹  Elisandra Rigo¹  Siu Mui Tsai²  Ademir Durrer²  Lillian Liva Foltran²  Marco Di Luccio²  J. Vladimir Oliveira²  Débora De Oliveira²  Helen Treichel² 

[1] ,Universidade Regional Integrada do Alto Uruguai e das Missões Departamento de Engenharia de Alimentos Erechim RS ,Brasil

关键词: Sporidiobolus pararoseus;    experimental design;    'synthetic lipase';    submerged fermentation;   

DOI  :  10.1590/S1517-83822012000400033

来源: SciELO

【 摘 要 】

The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (2(4-1)), yeast extract and NaCl concentrations were tested to further optimization by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold increase compared to the initial medium. The time course of 'synthetic lipase' production in the optimized condition was evaluated in terms of synthetic activity, protease activity, biomass and total carbon and the maximum synthetic activity was observed during the stationary phase of growth.

【 授权许可】

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