Computation of interactive effects and optimization of process parameters for alkaline lipase production by mutant strain of Pseudomonas aeruginosa using response surface methodology

Brazilian Journal of Microbiology

Deepali Bisht¹  Santosh Kumar Yadav¹  Nandan Singh Darmwal¹ 

[1] ,Dr. Ram Manohar Lohia Avadh University Department of Microbiology Center of ExcellenceFaizabad UP ,India

关键词: alkaline lipase;    Box-Behnken design;    Pseudomonas aeruginosa;    response surface methodology;   

DOI  :  10.1590/S1517-83822013005000016

来源: SciELO

【 摘 要 】

Alkaline lipase production by mutant strain of Pseudomonas aeruginosa MTCC 10,055 was optimized in shake flask batch fermentation using response surface methodology. An empirical model was developed through Box-Behnken experimental design to describe the relationship among tested variables (pH, temperature, castor oil, starch and triton-X-100). The second-order quadratic model determined the optimum conditions as castor oil, 1.77 mL.L-1; starch, 15.0 g.L-1; triton-X-100, 0.93 mL.L-1; incubation temperature, 34.12 ºC and pH 8.1 resulting into maximum alkaline lipase production (3142.57 U.mL-1). The quadratic model was in satisfactory adjustment with the experimental data as evidenced by a high coefficient of determination (R²) value (0.9987). The RSM facilitated the analysis and interpretation of experimental data to ascertain the optimum conditions of the variables for the process and recognized the contribution of individual variables to assess the response under optimal conditions. Hence Box-Behnken approach could fruitfully be applied for process optimization.

【 授权许可】

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