Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design

International Journal of Molecular Sciences

Wei Li¹  Li-Chun Zhao³  Yin-Shi Sun²  Feng-Jie Lei¹  Zi Wang¹  Xiong-Bin Gui³ 

[1] College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; E-Mails:;College of Agronomy, Shandong Agricultural University, Taian 271018, China; E-Mail:;The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China; E-Mail:

关键词: pressurized liquid extraction;    acetophenones;    Cynanchum bungei Decne;    Box-Behnken design;    response surface methodology;   

DOI  :  10.3390/ijms131114533

来源: mdpi

【 摘 要 】

In this work, pressurized liquid extraction (PLE) of three acetophenones (4-hydroxyacetophenone, baishouwubenzophenone, and 2,4-dihydroxyacetophenone) from Cynanchum bungei (ACB) were investigated. The optimal conditions for extraction of ACB were obtained using a Box-Behnken design, consisting of 17 experimental points, as follows: Ethanol (100%) as the extraction solvent at a temperature of 120 °C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 17 min. The extracted samples were analyzed by high-performance liquid chromatography using an UV detector. Under this optimal condition, the experimental values agreed with the predicted values by analysis of variance. The ACB extraction yield with optimal PLE was higher than that obtained by soxhlet extraction and heat-reflux extraction methods. The results suggest that the PLE method provides a good alternative for acetophenone extraction.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.