Expression of virulence genes by Listeria monocytogenes J0161 in natural environment

Brazilian Journal of Microbiology

Prem Saran Tirumalai¹  Soam Prakash¹ 

[1],Deemed University Dayalbagh Educational Institute Faculty of ScienceDayalbagh Agra ,India

关键词: Listeria monocytogenes;    prfA;    Virulence;    Microarray;    Biofilms;    Co-culture;    Gene Expression;   

DOI  :  10.1590/S1517-83822012000200050

来源: SciELO

【 摘 要 】

Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our objective was to study L.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a saprophytic to pathogenic form of L.monocytogenes. We, in this paper for the first time report the expression of prfA and other virulence genes by L.monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different from the previous reports. We also report that the expression of prfA seems to vary considerably when co-cultured with Bacillus subtilis.

【 授权许可】

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