Isolation and characterization of an Ashbya gossypii mutant for improved riboflavin production

Brazilian Journal of Microbiology

Shiping Wei²  James Hurley¹  Zhenglong Jiang²  Siwen Wang²  Yuanyuan Wang² 

[1] ,China University of Geosciences School of Marine Sciences Beijing,China

关键词: Riboflavin;    Ashbya gossypii;    Ultraviolet irradiation;    Medium optimization;   

DOI  :  10.1590/S1517-83822012000200003

来源: SciELO

【 摘 要 】

The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin production through dynamic analysis of its growth phase parameters, and riboflavin production could reach 8.12 g/l with pH was adjusted to the range of 6.0-7.0 using KH2PO4 in the later growth phase. This mutant has the potential to be used for industrial scale riboflavin production.

【 授权许可】

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