Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis

Brazilian Journal of Microbiology

Elisane Odriosolla Dos Santos¹  Mariano Michelon¹  Eliana Badiale Furlong²  Janaína Fernandes De Medeiros Burkert¹  Susana Juliano Kalil²  Carlos André Veiga Burkert¹ 

[1] ,Universidade Federal do Rio Grande Escola de Química e Alimentos Laboratório de Engenharia de BioprocessosRio Grande RS ,Brasil

关键词: Raw glycerol;    single cell protein;    yeast biomass;    Yarrowia lipolytica;   

DOI  :  10.1590/S1517-83822012000200002

来源: SciELO

【 摘 要 】

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License