Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis

Revista de microbiologia

Santos, Elisane Odriosolla dos¹ 

[1] Universidade Federal do Rio Grande, Rio Grande, Brasil

关键词: Raw glycerol;    single cell protein;    yeast biomass;    Yarrowia lipolytica.;   

DOI  :  10.1590/S1517-83822012000200002

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).

【 授权许可】

CC BY-NC