Bioconversion of ginsenoside Rb1 into compound K by Leuconostoc citreum LH1 isolated from kimchi

Brazilian Journal of Microbiology

Lin-hu Quan¹  Jin-ying Piao¹  Jin-woo Min¹  Dong-uk Yang¹  Hee Nyeong Lee¹  Deok Chun Yang¹ 

[1] ,Kyung Hee University Korean Ginseng Center Most Valuable Product and Ginseng Genetic Resource Bank College of Life Science Yongin,Korea

关键词: bioconversion;    compound K;    ginsenoside Rb1;    Leuconostoc citreum LH1;   

DOI  :  10.1590/S1517-83822011000300049

来源: SciELO

【 摘 要 】

About 40 different types of ginsenoside (ginseng saponin), a major pharmacological component of ginseng, have been identified along with their physiological activities. Among these, compound K has been reported to prevent the development of and the metastasis of cancer by blocking the formation of tumors and suppressing the invasion of cancerous cells. In this study, ginsenoside Rb1 was converted into compound K via interaction with the enzyme secreted by β-glucosidase active bacteria, Leuconostoc citreum LH1, extracted from kimchi. The optimum time for the conversion of Rb1 to compound K was about 72 hrs at a constant pH of 6.0 and an optimum temperature of about 30ºC. Under optimal conditions, ginsenoside Rb1 was decomposed and converted into compound K by 72 hrs post-reaction (99%). Both TLC and HPLC were used to analyze the enzymatic reaction. Ginsenoside Rb1 was consecutively converted to ginsenoside Rd, F2, and compound K via the hydrolyses of 20-C β-(1 → 6)-glucoside, 3-C β-(1 → 2)glucoside, and 3-C β-glucose of ginsenoside Rb1.

【 授权许可】

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