Mycoplasmas hyorhinis in different regions of cuba: diagnosis

Brazilian Journal of Microbiology

Evelyn Lobo²  Carlos Poveda¹  Alejandro Suarez¹  Yenney Hernández²  Ana Ramírez¹  José B. Poveda¹ 

[1] ,Laboratorio de Diagnóstico de Micoplasmas,Cuba

关键词: PCR;    mycoplasma;    diagnostic;    enzootic pneumonia;   

DOI  :  10.1590/S1517-83822011000200039

来源: SciELO

【 摘 要 】

M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic region 16S-23S of M. hyorhinis rRNA demonstrated the existing variability among strains of a same species. This study is the first report on M. hyorhinis detection in Cuba.

【 授权许可】

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