Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells

Brazilian Journal of Microbiology

Grimaldi Elena¹  Donnarumma Giovanna¹  Perfetto Brunella¹  De Filippis Anna¹  Melito Alessandro¹  Tufano Maria Antonietta¹ 

[1] ,Second University of Naples Faculty of Medicine and Surgery Department of Experimental Medicine, Microbiology and Clinical Microbiology Section,Italy

关键词: Porins;    TLRs;    PAMPs;    Shigella flexneri;    porinas;    TLR;    PAMP;    Shigella flexneri;   

DOI  :  10.1590/S1517-83822009000300036

来源: SciELO

【 摘 要 】

The recognition of bacterial components on the intestinal epithelial cells occurs through the toll-like receptors and is followed by the induction of an effective innate immune response. We analyzed receptor expression and signaling pathways involved in activation of human colon adenocarcinoma cells after stimulation with porins and LPS of Shigella flexneri. We also analyzed the expression and production of some cytokines, of intercellular adhesion molecule-1, of antimicrobial peptides human ²-defensins, and of the inducible form of nitric oxide synthase. Our data demonstrate that TLR2 is involved in porin recognition, whereas TLR4 with MD2, is required for LPS recognition.

【 授权许可】

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