Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191

Brazilian Journal of Microbiology

Luciana F. Fleuri¹  Haroldo Y. Kawaguti¹  Hélia H. Sato¹ 

[1] ,Universidade Estadual de Campinas, Campinas Faculdade de Engenharia de Alimentos Departamento de Ciências de AlimentosCampinas SP ,Brasil

关键词: chitinase;    Cellulosimicrobium cellulans;    fungal lysis;    protoplasts;    quitinase;    Cellulosimicrobium cellulans;    lise de fungos;    protoplastos;   

DOI  :  10.1590/S1517-83822009000300026

来源: SciELO

【 摘 要 】

This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.

【 授权许可】

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