Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus

Brazilian Journal of Microbiology

Fethi Ben Abdallah²  Kamel Chaieb²  Tarek Zmantar²  Hela Kallel¹  Amina Bakhrouf² 

[1] ,Faculté de Pharmacie Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits Monastir,Tunisia

关键词: Vibrio;    slime production;    adherence;    abiotic surface;    Vero cells;    Vibrio;    produção de muco;    adesão;    superfície abiótica;    células Vero;   

DOI  :  10.1590/S1517-83822009000200033

来源: SciELO

【 摘 要 】

In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains become adherent. The percentage of adherence ranged from1.23% to 4.66%.

【 授权许可】

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