Amiloride at pH 7.0 inhibits the Na+‐driven flagellar motors of Vibrio alginolyticus but allows cell growth

FEBS Letters

Atsumi, Tatsuo¹  Maekawa, Yukiyo¹  Imae, Yasuo¹  Tokuda, Hajime² 

[1] Department of Molecular, Biology, Faculty of Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan;Institute of Applied Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Na+-driven flagellar motor;    Vibrio;    Amiloride;   

DOI  :  10.1016/0014-5793(92)80954-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amiloride, a specific inhibitor for the Na⁺-driven flagellar motors of alkalophilic Bacillus, is known to inhibit secondarily the growth of alkalophiles. The motility of a marine Vibrio, V. alginolyticus, was almost completely inhibited by 2 mM amiloride either at pH 7.0 or 8.5. We found that this concentration of amiloride inhibited the cell growth completely at pH 8.5 but only slightly at pH 7.0. Kinetic analysis of the inhibition of motility by amiloride at pH 7.0 showed that the inhibition was competitive with Na⁺ in the medium. Thus, amiloride at pH 7.0 is really a specific and useful tool for the analysis of the Na⁺-driven flagellar motors of Vibro.

【 授权许可】

Unknown