Isolation and characterization of a novel strain of Stenotrophomonas maltophilia possessing various dioxygenases for monocyclic hydrocarbon degradation

Brazilian Journal of Microbiology

Guzik Urszula¹  Greń Izabela¹  Wojcieszyńska Danuta¹  Łabużek Sylwia¹ 

[1] ,University of Silesia Faculty of Biology and Environment Protection Department of BiochemistryKatowice,Poland

关键词: biodegradation;    Stenotrophomonas;    aromatic compounds;    dioxygenases;    biodegradação;    Stenotrophomonas;    compostos aromáticos;    dioxigenases;   

DOI  :  10.1590/S1517-83822009000200014

来源: SciELO

【 摘 要 】

A Gram-negative bacterium, designated as strain KB2, was isolated from activated sludge and was found to utilize different aromatic substrates as sole carbon and energy source. On the basis of morphological and physiochemical characteristics and 16S rRNA gene sequence analysis, the isolated strain KB2 was identified as Stenotrophomonas maltophilia. Strain KB2 is from among different Stenotrophomonas maltophilia strains the first one described as exhibiting the activities of three types of dioxygenases depending on the structure of the inducer. The cells grown on benzoate and catechol showed mainly catechol 1,2- dioxygenase activity. The activity of 2,3-dioxygenase was detected after phenol induction. Protocatechuate 3,4-dioxygenase was found in crude cell extracts of this strain after incubation with 4-hydroxybenzoic acid, protocatechuic acid and vanillic acid. Because of broad spectrum of dioxygenases' types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be very powerful and useful tool in the biotreatment of wastewaters and in soil decontamination.

【 授权许可】

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