Ligninases production by Basidiomycetes strains on lignocellulosic agricultural residues and their application in the decolorization of synthetic dyes

Brazilian Journal of Microbiology

Eleni Gomes¹  Ana Paula Aguiar¹  Caio César Carvalho¹  Maricy Raquel B. Bonfá¹  Roberto Da Silva¹  Mauricio Boscolo¹ 

[1] ,Universidade Estadual Paulista Instituto de Biociências, Letras e Ciências Exatas Laboratório de Bioquímica e Microbiologia AplicadaSão José do Rio Preto SP ,Brasil

关键词: laccase;    manganese peroxidase;    lignin peroxidase;    Basidiomycetes;    decolorization;    synthetic dye;    lacase;    manganês peroxidase;    lignina peroxidase;    Basidiomicetes;    descoloração;    corantes sintéticos;   

DOI  :  10.1590/S1517-83822009000100005

来源: SciELO

【 摘 要 】

Wood rotting Basidiomycetes collected in the "Estação Ecológica do Noroeste Paulista", São José do Rio Preto, São Paulo State, Brazil, concerning Aphyllophorales order and identified as Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp SXS48, Picnoporus sanguineus SXS 43 and Phellinus rimosus SXS47 were tested for ligninases production by solid state fermentation (SSF) using wheat bran or rice straw as culture media. C. byrsina produced the highest laccase (200 U mL-1) and Lentinus sp produced the highest activities of manganese peroxidase (MnP) and lignin peroxidase (LiP) (7 and 8 U mL-1, respectively), when cultivated on wheat bran. The effect of N addition on enzyme production was studied in medium containing rice straw and the data showed an increase of 3 up to 4-fold in the laccase production compared to that obtained in SSF on wheat bran. The laccases presented optimum pH at 3.0-3.5 and were stable at neutral pH values. Optimum pH for MnP and LiP activities was at 3.5 and between 4.5 and 6.0, respectively. All the strains produced laccase with optimum activities between 55-60°C while the peroxidases presented maximum activity at temperatures of 30 to 55°C. The crude enzymes promoted decolorization of chemically different dyes with around 70% of decolorization of RBBR and cybacron blue 3GA in 6h of treatment. The data indicated that enzymes from these basidiomycetes strains are able to decolorize synthetic dyes.

【 授权许可】

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