Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems

Brazilian Journal of Microbiology

M. Yamaguti²  E.e. Muller²  A.i. Piffer¹  J.d. Kich¹  C.s. Klein¹  S.s. Kuchiishi¹ 

[1] ,Universidade Estadual de LondrinaLondrina PR ,Brasil

关键词: Swine Enzootic Pneumonia;    Mycoplasma hyopneumoniae;    diagnosis;    Nested- PCR;    Pneumonia Enzoótica Suína;    Mycoplasma hyopneumoniae;    diagnóstico;    Nested-PCR;   

DOI  :  10.1590/S1517-83822008000300011

来源: SciELO

【 摘 要 】

Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport media, and DNA extraction methods, using eight pigs. Based on the optimization results, a second experiment was conducted for testing validity using 40 animals. In conclusion, the obtained results of the N-PCR optimization and validation allow us to recommend this test as a routine monitoring diagnostic method for Mycoplasma hyopneumoniae infection in swine herds.

【 授权许可】

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