Immune Response of Gnotobiotic Piglets against Mycoplasma hyopneumoniae

Journal of Veterinary Medical Science

Yoshihiro MUNETA²  Yu MINAGAWA²  Yohsuke OGAWA²  Hirokazu HIKONO²  Yoshihiro SHIMOJI²  Yasuyuki MORI¹ 

[1] Research Team for Paratuberculosis, National Institute of Animal Health;Research Team for Advanced Biologicals, National Institute of Animal Health

关键词: cytokine;    gnotobiotic;    immunomodulation;    Mycoplasma hyopneumoniae;    swine;   

DOI  :  10.1292/jvms.70.1065

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Cited-By(11)In this study, several cytokine responses were investigated during Mycoplasma hyopneumoniae (Mhp) infection using a gnotobiotic infection model. We found that several inflammatory cytokines (IL-1β, IL-8, IL-18, and TNF-α) and an anti-inflammatory cytokine IL-10 were induced from peripheral blood mononuclear cells (PBMC) of germ-free (GF) piglets stimulated with heat killed Mhp whole antigens, but no IFN-γ and IL-4 were induced by Mhp. After the intranasal infection of Mhp, IL-1β, IL-8, IL-18, and IFN-γ were also detected in the broncho-alveolar lavage fluids (BALF). The antigen-specific IFN-γ and IL-10 responses after infection of Mhp were gradually suppressed during Mhp infection as well as non-specific immune response to concanavalin A (ConA) and lipopolysacchalide (LPS) at early stage of infection. These results suggested that Mhp infection modulates the immune response of pigs by inducing several cytokines, and causes immuno-suppression of pigs in a gnotobiotic condition.

【 授权许可】

Unknown