Properties of an amylase from thermophilic Bacillus SP.

Brazilian Journal of Microbiology

Raquel Vieira De Carvalho¹  Thamy Lívia Ribeiro Côrrea¹  Júlia Caroline Matos Da Silva¹  Luciana Ribeiro Coutinho De Oliveira Mansur¹  Meire Lelis Leal Martins¹ 

[1] ,Universidade Estadual do Norte Fluminense Centro de Ciências e Tecnologias Agropecuárias Campos dos Goytacazes RJ ,Brasil

关键词: alpha-Amylase;    Thermophilic bacterium;    Bacillus sp;    alfa-Amilase;    Bactéria termofílica;    Bacillus sp;   

DOI  :  10.1590/S1517-83822008000100023

来源: SciELO

【 摘 要 】

alpha-Amylase production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid cultures containing soluble starch as a carbon source and supplemented with 0.05% whey protein and 0.2% peptone reached a maximum activity at 32 h, with levels of 37 U/mL. Studies on the amylase characterization revealed that the optimum temperature of this enzyme was 90ºC. The enzyme was stable for 1 h at temperatures ranging from 40-50ºC while at 90ºC, 66% of its maximum activity was lost. However, in the presence of 5 mM CaCl2, the enzyme was stable at 90ºC for 30 min and retained about 58% residual activity after 1 h. The optimum pH of the enzyme was found to be 8.5. After incubation of enzyme for 2 h at pH 9.5 and 11.0 was observed a decrease of about 6.3% and 16.5% of its original activity. At pH 6.0 the enzyme lost about 36% of its original activity. The enzyme was strongly inhibited by Co2+, Cu2+ and Ba2+, but less affected by Mg2+, Na+ and K+. In the presence of 2.0 M NaCl, 63% of amylase activity was retained after 2 h incubation at 45ºC. The amylase exhibited more than 70% activity when incubated for 1 h at 50ºC with sodium dodecyl sulphate. However, very little residual activity was obtained with sodium hypochlorite and with hydrogen peroxide the enzyme was completely inhibited. The compatibility of Bacillus sp SMIA-2 amylase with certain commercial detergents was shown to be good as the enzyme retained 86%, 85% and 75% of its activity after 20 min incubation at 50ºC in the presence of the detergent brands Omo®, Campeiro® and Tide®, respectively.

【 授权许可】

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