Characterization of protease from bacillus sp. on medium containing FeCl3 exposed to magnetic field 0.2 mt

3rd International Conference on Biological Sciences and Biotechnology

Sumardi^1 ; Agustrina, Rochmah^1 ; Ekowati, Christina Nugroho^1 ; Pasaribu, Yovita Selvie^1

Department of Biology, Faculty Mathematic and Natural Science, University of Lampung, Jalan Prof. Dr. Soemantri Brodjonegoro No. 1, Bandar Lampung, Lampung

35145, Indonesia^1

关键词: Bacillus sp;    determine;    Exposed to;    inductor;    Media content;    Protease enzyme;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012046/pdf
DOI  :  10.1088/1755-1315/130/1/012046

来源: IOP

【 摘 要 】

This purpose of this research is to determine the character of the protease enzymes from Bacillus sp. on media content of FeCl3exposed to 0.2 mT magnetic field. The data obtained were analyzed descriptively. The result showed that protease enzyme without Fe resulted in the highest activity at pH 8, temperature. 30°C with the addition of activator Mn2+, and Vmaxof 0.28 U/ml, and Kmof 4.60 U/ml. The protease enzyme on media without magnetic field exposure and containing Fe yielded the highest activity at pH 8, temperature 30°C with the addition of activator Mn2+, and Vmaxof 0.33 U/ml, and Kmof 5.64 U/ml. The protease enzyme on medium with magnetic field exposure and use Fe as inductors have the highest activity at pH 9, the temperature of 55° C with the addition of activator Mn2+, and Vmaxof 0.35 U/ml, and Km 10.04 U/ml.