Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions

Brazilian Journal of Microbiology

Renata Yamanaka²  Clarissa F. Soares²  Dácio R. Matheus¹  Kátia M.g. Machado² 

[1] ,Universidade Católica de SantosSantos SP ,Brasil

关键词: laccase;    metals;    MnP;    vegetable oil;    surfactants;    lacase;    metais;    MnP;    óleo vegetal;    surfactante;   

DOI  :  10.1590/S1517-83822008000100019

来源: SciELO

【 摘 要 】

The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics, mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within the first days of growth. Laccase was the main activity detected under the different conditions and was produced throughout the culture period of the fungus, irrespective of the growth phase. Supplementation of the medium with vegetable oil emulsified with a surfactant induced manganese-dependent peroxidase activity in T. villosa. Higher specific yields of laccase activity were obtained with the addition of copper.

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