Extended-spectrum beta-lactamase production among ampicillin-resistant Escherichia coli strains from chicken in Enugu State, Nigeria

Brazilian Journal of Microbiology

K.f. Chah¹  S.i. Oboegbulem² 

[1] ,Department of Veterinary Pathology and Microbiology

关键词: Ampicillin-resistant;    Escherichia coli;    beta-lactamase;    extended-spectrum;    Escherichia coli;    resistência a ampicilina;    beta-lactamase;    espectro expandido;   

DOI  :  10.1590/S1517-83822007000400018

来源: SciELO

【 摘 要 】

One hundred and seventy-two ampicillin-resistant E. coli strains isolated from commercial chickens in Enugu State, Nigeria, were screened for beta-lactamase production using the broth method with nitrocefin® as the chromogenic cephalosporin to detect enzyme production. Beta-lactamase producing strains were further examined for extended-spectrum beta-lactamase (ESBL) production using the Oxoid combination discs method. One hundred and seventy (98.8%) of the 172 ampicillin-resistant E. coli strains produced beta-lactamase enzyme. Sixteen (9.4%) beta-lactamase producers were phenotypically confirmed to produce ESBLs. Six of the ESBL producing strains were only detected with ceftazidime versus ceftazidime/clavulanate combination while ten of the ESBL producers were detected with cefotaxime versus cefotaxime/clavulanate combination. Chicken may serve as a reservoir of ESBL-producing E. coli strains which could be transferred to man and other animals.

【 授权许可】

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