Analysis of the in vitro adherence of Streptococcus mutans and Candida albicans

Brazilian Journal of Microbiology

Dicler De Sant'anna Vitor Barbieri²  Vânia Aparecida Vicente²  Fabian Calixto Fraiz¹  Osmir José Lavoranti¹  Terezinha Inês Estivalet Svidzinski¹  Rosângela Lameira Pinheiro¹ 

[1] ,Universidade Federal do Paraná Departamento de Patologia Básica Curitiba PR ,Brasil

关键词: Adherence;    Candida albicans;    Streptococcus mutans;    biofilm;    Aderência;    Candida albicans;    Streptococcus mutans;    biofilme;   

DOI  :  10.1590/S1517-83822007000400009

来源: SciELO

【 摘 要 】

The objective of the present study was to investigate the in vitro adherence capacity of Streptococcus mutans and Candida albicans. Adherence assays were conducted on dental surfaces and analyzed by scanning electron microscopy (SEM). Extracted human teeth were inoculated with Streptococcus mutans or Candida albicans and with both species simultaneously, and incubated at 37ºC for 21 days. Bacterial inocula had been obtained from saliva samples of children that had been colonized by both organisms. ATCC reference strains were used as controls. SEM analyses showed that the biofilm that covered the entire analyzed dental surface was more homogeneous inoculated with the two microorganisms simultaneously than with each species separately. In a second experiment, carried out with isolates that had shown the highest adherence the isolates were tested for adherence to high-density polyethylene substrates. The potentialization of the adherence capacity of Streptococcus mutans and Candida albicans when in association was confirmed.

【 授权许可】

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