Virus isolation and molecular characterization of canine distemper virus by RT-PCR from a mature dog with multifocal encephalomyelit

Brazilian Journal of Microbiology

Alexandre Mendes Amude¹  Glei Dos Anjos Carvalho¹  Amauri Alcindo Alfieri¹  Alice Fernandes Alfieri¹ 

[1] ,Universidade Estadual de Londrina Centro de Ciências Agrárias Departamento de Medicina Veterinária PreventivaLondrina PR ,Brasil

关键词: dog;    canine distemper virus;    encephalomyelitis;    RT-PCR;    cão;    vírus da cinomose canina;    encefalomielite;    RT-PCR;   

DOI  :  10.1590/S1517-83822007000200031

来源: SciELO

【 摘 要 】

A case of multifocal distemper encephalomyelitis in a mature dog is described. In the presented case the ante mortem clinical diagnosis of canine distemper virus (CDV) infection could not be ideally performed due to the absence of typical signs of distemper, such as myoclonus and systemic signs accompanying the nervous signs. The definitive diagnosis of distemper encephalomyelitis was only carried out at post mortem through virus isolation in cell culture from fresh central nervous system (CNS) fragments and CDV nucleoprotein gene detection in the CNS by RT-PCR.

【 授权许可】

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