Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Brazilian Journal of Microbiology

Patrícia Gomes Cardoso¹  Marisa Vieira De Queiroz¹  Olinto Liparini Pereira¹  Elza Fernandes De Araújo¹ 

[1] ,Universidade Federal de Viçosa BIOAGRO Departamento de MicrobiologiaViçosa MG ,Brazil

关键词: Ribossomal DNA;    Internal Transcribed Spacer;    Subtelomeric Region;    Penicillium spp;    DNA ribossômico;    Espaçador Transcrito Interno;    Região Subtelomérica;    Penicillium spp;   

DOI  :  10.1590/S1517-83822007000100015

来源: SciELO

【 摘 要 】

Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. The polymorphism observed in the organization of the subtelomeric region in the genome of two Penicillium species within the high homogeneous Penicillium subgenus is for the first time reported and perhaps can be employed in future phylogenetic studies.

【 授权许可】

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