Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil

Brazilian Journal of Microbiology

Terezinha Knöbl¹  Tânia Aparecida Tardelli Gomes²  Mônica Aparecida Midolli Vieira²  Fernando Ferreira²  José Américo Bottino²  Antônio José Piantino Ferreira² 

[1] ,Faculdades Metropolitanas Unidas Faculdade de Medicina Veterinária São Paulo SP ,Brasil

关键词: APEC;    Escherichia coli;    adhesins;    colibacillosis;    poultry;    APEC;    Escherichia coli;    adesinas;    colibacilose;    ave;   

DOI  :  10.1590/S1517-83822006000300033

来源: SciELO

【 摘 要 】

Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae probes. The adherence to chicken tracheal cells showed 96% positive strains, while hemagglutination assays showed 26.5% of the isolates were mannose sensitive and 21.7% were mannose resistant.

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