Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans

Brazilian Journal of Microbiology

Fernanda Akemi Nakanishi²  Mario Julio Avila-campos¹  Nádia Hizuru Kamiji¹  Eiko Nakagawa Itano¹ 

[1] ,Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão Paulo SP ,Brasil

关键词: immunoglobulin G;    Actinobacillus actinomycetemcomitans;    ELISA;    protease;    imunoglobulina G;    Actinobacillus actinomycetemcomitans;    ELISA;    protease;   

DOI  :  10.1590/S1517-83822006000100008

来源: SciELO

【 摘 要 】

Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The molecular mass of the protease active fraction was from 43 to 150 kDa.

【 授权许可】

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