LEUKOTOXIC ACTIVITY OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS ISOLATED FROM HUMAN AND NON-HUMAN PRIMATES

Brazilian Journal of Microbiology

Francisca Lúcia De Lima²  Flávio Furtado De Farias¹  Patrícia Cota Campos¹  Antônio Helvécio Totola¹  Carlos Alberto Pereira Tavares¹  José Eustáquio Da Costa¹  Luiz De Mácêdo Farias¹  Maria Auxiliadora Roque De Carvalho¹ 

[1] ,Universidade Estadual do Piauí Centro de Ciências Biológicas e da Saúde Teresina PI ,Brasil

关键词: Actinobacillus actinomycetemcomitans;    periodontopathogen;    leukotoxic activity;    chemiluminescence assay;    Actinobacillus actinomycetemcomitans;    periodontopatógeno;    atividade eucotóxica;    ensaio de quimioluminescência;   

DOI  :  10.1590/S1517-83822001000300018

来源: SciELO

【 摘 要 】

Actinobacillus actinomycetemcomitans is a clinically relevant periodontopathogenic Gram-negative coccobacillus that produces a leukotoxin of the RTX cytolysin family. In this study, we evaluated the leukotoxic activity of A. actinomycetemcomitans strains isolated from human and marmosets by Trypan blue exclusion and by the chemiluminescence assays. Among eight A. actinomycetemcomitans human strains studied, two (P2.17 and P8.12) were classified as high leukotoxin producers and among eight marmoset strains, one (M22.11) showed high leukotoxin production, as determined by Trypan blue exclusion assay. The reference strains ATCC 29523 and FDC Y4 respectively behaved like moderate and low producers. The chemiluminescence assay was used to evaluate the leukotoxic activity of M22.11 and P2.17 strains submitted to different growth conditions. Leukotoxic activity was detected on cells at the logarithmic phase and was similar under anaerobic and microaerophilic growth conditions. It was greatly reduced when cells were grown at glucose concentrations lower or higher than 0.75% (0.25% and 1.5%) in thioglycolate medium. Leukotoxin production mainly by the M22.11 strain was low in BHI broth, whereas production in TSB medium showed a similar level as in thioglycolate broth medium. Sodium bicarbonate at 10 mM did not affect leukotoxin production.

【 授权许可】

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