Production and caracterization of monoclonal antibodies for the detection of Salmonella enterica in chicken meat

Brazilian Journal of Microbiology

Andréa Dos Santos Schneid¹  Charli Beatriz Lüdtke¹  Cristiane Diel¹  José Antonioguimarães Aleixo¹ 

[1] ,Universidade Federal de Pelotas Centro de Biotecnologia Pelotas RS ,Brasil

关键词: Salmonella;    monoclonal antibodies;    membrane proteins;    foods;    Salmonella;    anticorpos monoclonais;    proteinas de membrana;    alimentos;   

DOI  :  10.1590/S1517-83822005000200012

来源: SciELO

【 摘 要 】

A panel of 13 monoclonal antibodies (MAbs) that react against outer membrane proteins of Salmonella Enteritidis was obtained. Two MAbs were classified as IgM, six were IgG2a, three were IgG3 and one was of the IgG2b isotype. The reactivity of the MAbs against different serovars of Salmonella enterica and other bacteria was investigated using an indirect ELISA. Five MAbs reacted only against Salmonella Enteritidis. Two MAbs presented crossed reactions with thermo-extracted antigens of Klebsiella pneumoniae, Citrobacter freundii and Enterobacter aerogenes. MAb 424H presented wide spectrum of reactivity, detecting antigens of Salmonella belonging to serogroups B, C, D, E and G. The detection limit of different serovars of Salmonella in a indirect ELISA with MAb 424H varied from 1.0 x 10(4) CFU/mL for Salmonella London to 1.4 x 10(6) CFU/mL for Salmonella Gallinarum and Salmonella Typhimurium. Evaluation of the performance of the ELISA with MAb 424H in the detection of Salmonella in samples of chicken meat artificially contaminated revealed that the ELISA was able to detect all serovars after sample enrichment using two levels of contamination. Samples of chicken meat not artificially contaminated analysed in parallel were negative for Salmonella in both the conventional and the ELISA methods.

【 授权许可】

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