Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers

Brazilian Journal of Microbiology

Bereneuza Tavares Ramos Valente Brasileiro²  Maria Raquel Moura Coimbra¹  Marcos Antonio De Morais Jr¹  Neiva Tinti De Oliveira² 

[1] ,Universidade Federal de Pernambuco Departamento de Micologia Recife PE ,Brasil

关键词: intron splice site;    Fusarium solani;    microsatellite;    PCR-fingerprinting;    ribosomal DNA;    DNA ribossomal;    intron splice site;    Fusarium solani;    impressão genética por PCR;    microssatélite;   

DOI  :  10.1590/S1517-83822004000200006

来源: SciELO

【 摘 要 】

Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical origin and substrate. Its polymorphism was observed even in the very conserved sequence of rDNA locus, and the SPAR marker (GTG)5 showed the highest polymorphism. Together, those results may contribute to understand the relation between fungal genetic variability and cultivars resistance phenotypes to fungal-caused diseases, helping plant-breeding programs.

【 授权许可】

CC BY-NC   
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