Laryngotracheitis: reproducibility of the disease and comparison of diagnostic methods

Brazilian Journal of Microbiology

Nilzane Beltrão²  Thales Quedi Furian²  Guilherme Fonseca De Souza²  Marisa Macagnan²  Luiz César Bello Fallavena¹  Claudio Wageck Canal² 

[1] ,Universidade Federal do Rio Grande do Sul Faculdade de Veterinária Centro de Diagnóstico e Pesquisa em Patologia AviáriaPorto Alegre RS ,Brasil

关键词: infectious laryngotracheitis virus;    avian pathology;    diagnosis;    vírus da laringotraqueíte infecciosa;    patologia aviaria;    diagnóstico;   

DOI  :  10.1590/S1517-83822003000500024

来源: SciELO

【 摘 要 】

Infectious laryngotracheitis virus (ILTV) cause mild to severe respiratory disease in chickens, the purpose of our study being to use Brazilian isolate of ILTV to reproduce ILTV disease in chickens by experimental infection and to compare three diagnostic methods (nested polymerase chain reaction (PCR), virus isolation, histopathology) for detection of ILTV. Forty-eight chickens intratracheally inoculated with ILTV and a further 48 with PBS, showing mild respiratory signs 48 hours post infection (PI) but no signs of infection after day 10 PI. Every 2 days PI, six birds were arbitrarily selected from the control and infected groups, sacrificed and the trachea collected. Both the nested PCR and virus isolation detected the virus from day 2 until day 12 PI. However, at day 12 PI, PCR detected ILTV DNA in 100% of the samples while the virus isolation method detected ILTV in only 33% of the samples. Tracheal histopathology showed intranuclear inclusion bodies on days 8 and 10 PI. The results indicate that the field-isolate of ILTV studied by us is of low pathogenicity and that our nested PCR protocol was able to detect positive samples over a longer infection period than many ILTV diagnostic test already described.

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